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KMID : 1134820100390020193
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Journal of the Korean Society of Food Science and Nutrition
2010 Volume.39 No. 2 p.193 ~ p.202
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Antioxidative Activities of the Codonopsis lanceolata Extract in vitro and in vivo
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Kim Soo-Hyun
Chung Mi-Ja
Jang Hae-Dong
Ham Seung-Shi
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Abstract
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In vitro activities of Codonopsis lanceolata (CL) 70% ethanol extract and its fractions (hexane, chloroform, ethyl acetate, butanol and water) were examined by total polyphenol content, reducing power, 2,2’-azino-bis(3-ethylbenzothiazoline-6-sulfonate) (ABTS), 2,2-diphenyl-β-picrylhydrazyl (DPPH), and oxygen radical absorbance capacity (ORAC) assays. The ethyl acetate fraction from CL ethanol extract (CLEA) showed the highest total polyphenol content (22.7 ㎎/g) among five fractions, and also exhibited an excellent reducing power (0.42~1.27 at 250~1,000 ㎍/㎖). CLEA at 100~400 ㎍/㎖ concentrations had 27.7~70.3% of ABTS radical scavenging activity and the highest DPPH radical scavenging activity (81.6% at 400 ㎍/㎖). CLEA had dominantly higher ORACROOㆍ activity compared to other fractions. CLEA and butanol fraction had significantly higher ORACOHㆍ activities than 70% ethanol extract, hexane, chloroform and water fractions. The CLEA exhibited the highest antioxidant activity in CL 70% ethanol extract and its fractions. Thus, effect of CLEA treatment on antioxidant gene expression under the oxidative stress conditions by a high fat diet in animal model was studied by microarray and RT-PCR methods. The 31 antioxidant genes were expressed but the genes were not up-regulated at least a two-fold by CLEA treatment. We concluded that CLEA does not have an indirect antioxidant effect but a direct antioxidant effect by up-regulation of antioxidant genes in high fat diet-induced obese mice.
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KEYWORD
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antioxidant activity, antioxidant gene, Codonopsis lanceolata, microarray, oxidative stress
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